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Scirj Volume III, Issue II, February 2015 Edition
ISSN: 2201-2796

Investigating Lipase Activity in Ungerminated Colocynthis citrullus lanatus (Egusi Melon) Seeds

Jonathan Bege, Markus Victor, Gaiya Daniel

Abstract: Activity of lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) was investigated in ungerminated Colocynthis citrullus lanatus (Egusi Melon) seeds. Titrimetric analytical method with 0.01 M NaOH using phenolphthalein as indicator was the method employed. The lipase activity was expressed as the percent free fatty acids (FFAs) liberated. The results obtained showed that ungerminated seeds of Egusi melon (Colocynthis citrullus lanatus) have lipase activity. It was observed that 1g of the enzyme preparation was enough to optimally act on 5g oil in 2.5ml hexane. The optimum conditions for lipolysis were found to be 30oC and 60 min period of incubation. This shows that Colocynthis citrullus lanatus seeds will deteriorate with storage. The egusi melon seed lipase could be exploited commercially for industrial applications.

Reference this Paper: Investigating Lipase Activity in Ungerminated Colocynthis citrullus lanatus (Egusi Melon) Seeds by Jonathan Bege, Markus Victor, Gaiya Daniel published at: "Scientific Research Journal (Scirj), Volume III, Issue II, February 2015 Edition, Page 35-38 ".

Search Terms: Colocynthis citrullus lanatus, ungerminated seed, lipase activity

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