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Scirj, Volume V [2017]
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Scirj Volume V, Issue XII, December 2017 Edition
ISSN: 2201-2796

Isolation and Characterization of Phytase-Producing thermophilic Bacteria from Sulili Hot Springs in South Sulawesi

Hafsan, Ilham Ibnu Irwan, L Agustina, A Natsir, A Ahmad

Abstract: This study aimed to isolate and select and identify phytase-producing thermophilic bacteria from Sulili hot springs in the district Pinrang South Sulawesi, Indonesia. water and sediment samples were obtained from Sulili hot springs the district Pinrang, South Sulawesi. Isolation was done using Luria Bertani (LB) medium at 60°C for 1 x 24 hours. The twelve isolates of thermophilic bacteria have been obtained, five of them have the ability to produce phytase. Signed by the formation of a clear zone around the colonies grown on medium screening LB + Ca-phytate. Five isolates were then selected, three of whom are selected based isolates widest phytase activity qualitatively. Morphological characteristics and biochemical nature of the three selected isolates have indicated belong to genera of Bacillus. Each has likeness with Bacillus licheniformis, Bacillus coagulans and Bacillus stearothermophillus from Sulili hot springs have obtained three isolates of thermophilic bacteria showed likeness with Bacillus licheniformis, Bacillus coagulans and Bacillus stearothermophillus.

Reference this Paper: Isolation and Characterization of Phytase-Producing thermophilic Bacteria from Sulili Hot Springs in South Sulawesi by Hafsan, Ilham Ibnu Irwan, L Agustina, A Natsir, A Ahmad published at: "Scientific Research Journal (Scirj), Volume V, Issue XII, December 2017 Edition, Page 16-19 ".

Search Terms: Sulili hot springs, Phytase, thermophilic bacteria, Bacillus

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