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Scirj Volume II, Issue IV, April 2014 Edition
ISSN: 2201-2796

The Demonstration of Larval Polytene Chromosomes of Anopheles Gambiae

AJU-AMEH

Abstract: Anopheles gambiae (Diptera: Culicidae) larvae were found in large numbers in the open ground pools at Rayfield, Jos and a few from along the stream edges formed by some pool. Within the pool mixed populations of both culicine and Anopheline larvae were found. A total of four hundred and ninety-seven Anopheline larvae were brought into the insectary from the wild. Four hundred and forty-four were reared to adult stage successfully. Fifty were fixed for dissection and three died. Laboratory colonization of the Anopheles gambie failed due to, among other factors, that they could not blood feed. Consequently only the salivary gland polytene chromosome squashes of wild caught larvae were examined. Dissections of the salivary gland of the Anopheles yielded chromosomes which spread successfully as expected. A Least Significance Difference test (LSD) showed a highly significant interaction between seasons and sites.

Reference this Paper: The Demonstration of Larval Polytene Chromosomes of Anopheles Gambiae by AJU-AMEH published at: "Scientific Research Journal (Scirj), Volume II, Issue IV, April 2014 Edition, Page 23-29 ".

Search Terms: Anopheles gambiae, Larval instar, Polytene chromosomes,Salivary gland

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