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Scirj, Volume VIII [2020]
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Scirj Volume VIII, Issue IV, April 2020 Edition
ISSN: 2201-2796

Production, Purification and Characterisation of Keratinases From Bacillus species Isolated From Poultry Feather Waste

Michael Dada, Sherifah Wakil

Abstract: Feather is high in protein (keratin) and is not easily degradable in nature. It is produced in large amounts as waste through poultry activities all over the world. In order to provide sufficient use for this keratin-rich waste the keratin present in feather can serve as keratinase inducer. Hence this study focuses on the production, purification and characterisation of keratinases from isolated keratin-degrading Bacillus licheniformis-K51, Bacillus subtilis-K50 and Bacillus sp.-K53. Bacillus licheniformis-K51 gave highest keratinolytic activity (24.76±0.91 U/mL) at pH 7.8, 37 oC, agitation rate 150 rpm and 0.3% (NH4)2SO4 on day 7, while Bacillus subtilis-K50 had 19.03±0.74 U/mL at pH 7.5, 37 oC, agitation rate 200 rpm and 1.4% cellulose on day 4. Bacillus sp.-K53 gave least activity (18.41±0.60 U/mL) at pH 7.2, 37 oC and agitation rate 150 rpm on day 5. Molecular mass of purified keratinases was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis and was obtained between 33-36 kDa. Purified enzyme of Bacillus licheniformis-K51 (EZYKer-51) contained Glutamate (18.2%), Alanine (14.9%) and showed highest activity (26.31 U/mL) at 60 oC and pH 8 with KM and Vmax kinetic constants of 25.60 mM and 74.46 U/mL, respectively.

Reference this Paper: Production, Purification and Characterisation of Keratinases From Bacillus species Isolated From Poultry Feather Waste by Michael Dada, Sherifah Wakil published at: "Scientific Research Journal (Scirj), Volume VIII, Issue IV, April 2020 Edition, Page 83-98 ".

Search Terms: Keratin-degrading bacteria, Bacillus keratinases, Keratin substrate, optimization

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