Anti-mycobacterium activity and Bioassay guided fractionation of the leave extract of Ximenia Americana L.

Dublin Core

Title

Anti-mycobacterium activity and Bioassay guided fractionation of the leave extract of Ximenia Americana L.

Description

This work was aimed at providing preliminary findings that unraveled the promising potential of X. americana for the management of Mycobacterium bovis. The study tried to assess the anti-mycobacterial activity of Ximenia americana using the leave extract after extracting with methanol for 48 hours and the extract subjected to serial solvent partitioning with hexane, chloroform and n-butanol. The various solvent fractions obtained from the partitioning were screened against Mycobacterium bovis and the result showed hexane, chloroform and n-butanol extracts to inhibit the growth of the bacteria with a minimum concentration (MIC) of 3200 µg/ml, 1800 µg/ml and 3500 µg/ml respectively. The activity of the chloroform extract was found to be higher than the hexane and n-butanol as such Bio-assay guided fractionation of the chloroform extract was further carried out using column chromatography and nine fractions were obtained. The fourth and Fifth fractions showed strongest activity at MICs of 130 µg/ml and 140 µg/ml. The mixture of these two most active components gave an improved minimum inhibitory concentration of 78 µg/ml fraction after preparative thin layer chromatography.

Creator

Nvau J. B., Auwal M., Nangbes J. G., Wufem B.M.

Source

Scirj Volume II, Issue VI, June 2014 Edition

Publisher

Scientific Research Journal (Scirj)
ISSN: 2201-2796

Date

26 Aug 2014

Identifier

http://www.scirj.org/jun-2014-paper.php?rp=P0614144

Citation

Nvau J. B., Auwal M., Nangbes J. G., Wufem B.M. , “Anti-mycobacterium activity and Bioassay guided fractionation of the leave extract of Ximenia Americana L. ,” Scientific Research Journal -> Research Library, accessed November 19, 2017, http://www.scirj.org/rp/items/show/84.

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