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Scirj Volume IV, Issue III, March 2016 Edition ISSN: 2201-2796 Detection of virulence genes in urinary Pseudomonas aeruginosa from pregnant women attending Antenatal clinicin Makurdi, Central Nigeria Aernan, P.T; Umeh, E.U and Emmanuel Nna Abstract: Pseudomonas aeruginosais known to possess a vast array of virulence factors that may be responsible for its pathogenicity. P. aeruginosa also has a large number of virulence factors including phospholipase C, both of the hemolytic and non- hemolytic type encoded by plc H and plc N gene respectively. Exotoxin A, encoded by the tox A gene,exoenzyme S, encoded by the exo S gene, Las B elastase encoded by the las B gene and Alginate encoded by the alg D gene. The aim of this study was to determine the prevalence of these six virulence genes; (plc H, plc N, tox A, exo S, las B, and alg D) by Polymerase Chain Reaction (PCR). In this study 28 isolates of P. aeruginosa were isolated from the urine of pregnant women attending antenatal in a tertiary hospital in Makurdi, Central Nigeria.The amplification of the total DNA extracts using singleplex polymerase chain reaction detected alg D in 2 (7.14%), Las Bin 3 (10.7%), tox A in 9(32.2%), plc H in 1(3.6%) and plc Nin 5(17.9%) of the samples. The exo S gene was not detected in the genome of the Pseudomonas aeruginosa bacteria isolates. Determination of different virulence genes of P. aeruginosa isolates suggests that they are associated with different levels of intrinsic virulence and pathogenicity.The presence of virulent strains of P. aeruginosa within the urinary tract of pregnant women calls for concern as this can contribute to development of complications associated with pyelonephritis, which may in turn lead to adverse outcomes for the baby and the mother, such as premature delivery, low birth weight infants, preeclampsia, hypertension, renal failure and fetal death. Reference this Paper: Detection of virulence genes in urinary Pseudomonas aeruginosa from pregnant women attending Antenatal clinicin Makurdi, Central Nigeria by Aernan, P.T; Umeh, E.U and Emmanuel Nna published at: "Scientific Research Journal (Scirj), Volume IV, Issue III, March 2016 Edition, Page 57 - 65 ". Search Terms: Virulence, Pseudomonas aeruginosa, pregnant women, Central Nigeria [Read Research Paper] [Full Screen] |
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